Double Isotope Derivative Assay of Aldosterone in Biological Extracts
نویسندگان
چکیده
منابع مشابه
Double isotope derivative assay of aldosterone in biological extracts.
The selective measurement of aldosterone in biological fluids has been handicapped by the lack of specific and sensitive assays. Bioassay methods (l-4) have been limited by the variation inherent in animal response. Chemical methods (5-9) require extensive purification procedures to remove contaminants, and are therefore subject to variable recoveries of the steroid. Furthermore, the quantity o...
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1. Two techniques for estimating plasma aldosterone concentration are compared by means of repeated assays of a plasma pool and also by analysis of a wide range of plasma samples. 2. No significant difference was found in the results obtained by the methods. Radioimmunoassay required only one tenth of the volume of plasma needed for the double-isotope derivative method. 3. Its rapidity and rela...
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Although the cardiac glycosides have been used extensively since Withering published his observations on the effects of foxglove nearly two hundred years ago (1), their distribution and metabolism and the cellular basis of their action in man are unknown. The lack of sufficiently sensitive, chemically specific methods of assay has hampered studies of the biologic behavior of these compounds. Th...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1960
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)76855-0